mcdhf diet a06071309  (research diets inc)

Journal: Frontiers in Immunology

Article Title: Activated Neutrophils Secrete Chitinase-Like 1 and Attenuate Liver Inflammation by Inhibiting Pro-Inflammatory Macrophage Responses

doi: 10.3389/fimmu.2022.824385

Figure Lengend Snippet: Infusion of A-Neu ameliorated MCDHF-induced liver injury in mice. (A) Schematic diagram of experimental design of tail vein injection of neutrophils (5×10 6 cells/mouse) after 2 weeks of MCDHF diet in mice. (B) Representative flow cytometry analysis of EGFP + Ly6G + neutrophils on CD45 + CD11b + monocytes in the liver of mice in 4 hours or 12 hours after injection of EGFP + N-Neu or EGFP + A-Neu. (C) Infusing N-Neu or A-Neu into mice after 2 weeks of MCDHF diet and harvesting liver in 4 days after injection. The representative H&E staining images of liver sections were performed, inflammatory area was indicated by circle. (D) Areas of Inflammation were quantified, (control group, n=3; MCDHF group, n=6). (E) Serum ALT levels were measured in indicated groups, n=5 each group. The results were presented as mean ± SD. One-way ANOVA was used. *P < 0.05. Scale bar = 100μm. MCDHF, methionine-choline-deficient and high-fat diet; A-Neu, activated neutrophil; N-Neu, non-activated neutrophil; ALT, alanine aminotransferase; EGFP, enhanced green fluorescence protein.

Article Snippet: To induce liver inflammation, mice were fed with control diet or MCDHF diet (A06071309, Research Diet Inc, NJ, USA), an L-amino acid diet containing 45 kcal of fat, 0.1% methionine, and no added choline.

Techniques: Injection, Flow Cytometry, Staining, Control, Fluorescence

Journal: Frontiers in Immunology

Article Title: Activated Neutrophils Secrete Chitinase-Like 1 and Attenuate Liver Inflammation by Inhibiting Pro-Inflammatory Macrophage Responses

doi: 10.3389/fimmu.2022.824385

Figure Lengend Snippet: A-Neu inhibited the expression of pro-inflammatory cytokines in the injured liver of mice induced by MCDHF. (A) N-Neu or A-Neu were injected into mice 2 weeks after MCDHF diet, and the livers were collected at 4 days after injection. Hepatic expressions of Ccl4 , Tnf and Nos2 transcripts were measured by RT-qPCR, presented relative to Gapdh , (control group, n=4; MCDHF group, n=6). The representative scatter plot of CBA, as shown in (B) , and the two bead populations represented CCL4 and TNF respectively. (C) Showed the quantification of these protein concentrations (control group, n=3; MCDHF group, n=7). (D) NOS2 protein levels in indicated groups were examined by WB (n=6). The results were presented as mean ± SD. One-way ANOVA was used. *P < 0.05. MCDHF, methionine-choline-deficient and high-fat diet; A-Neu, activated neutrophil; N-Neu, non-activated neutrophil; Ccl4, C-C chemokine motif ligand 4; Tnf, tumor necrosis factor; Nos2, nitric oxide synthase 2.

Article Snippet: To induce liver inflammation, mice were fed with control diet or MCDHF diet (A06071309, Research Diet Inc, NJ, USA), an L-amino acid diet containing 45 kcal of fat, 0.1% methionine, and no added choline.

Techniques: Expressing, Injection, Quantitative RT-PCR, Control